References
Andersson, B.; Vidal, R.; Sundberg, M.; Rajs, J.; Sótonyi, P. Hydroperoxide-induced nculeotide degradation and adenosine formation in isolated rat cardiomyocytes. Toxicology 106: 39–48; 1996.
Bhatnagar, A. Biochemical mechanism of irreversible cell injury caused by free radical-initiated reactions. Mol. Cell. Biochem. 137(1): 9–16; 1994.
Castro, G. J.; Bhatnagar, A. Effect of extracellular ions and modulators of calcium transport on survival of tert-butyl hydroperoxide exposed cardiac myocytes. Cardiovasc. Res. 27 (10): 1873–1881; 1993.
Cazorla, O.; Pascarel, C.; Garnier, D.; Guennec, J. Y. L. Resting tenstion participates in the modulation of active tension in isolated guniea pig ventricular myocytes. J. Mol. Cell. Cardiol. 29 (6): 1629–1637; 1997.
Daly, M. J.; Young, R. J.; Britnell, S. L.; Nayler, W. G. The role of calcium in the toxic effects of tert-butyl hydroperoxide on adult rat cardiac myocytes. J. Mol. Cell. Cardiol. 23: 1303–1312; 1991.
Eklow, L.; Moldéus, P.; Orrenius, S. Oxidation of glutathione during hydroperoxide metabolism. A study using isolated hepatocytes and the glutathione reductase inhibitor 1,3-bis(2-chloroethyl)-1-nitrosourea. Eur. J. Biochem. 138: 459–463; 1984.
El-Shenawy, N.; Abdel-Rahman, M. Evaluation of chloroform cardiotoxicity utilizing a modified isolated rat cardiac myocytes. Toxicol. Lett. 69: 249–256; 1993.
Fernandes, E.; Carvalho, E.; Remião, F.; Bastos, M. L.; Pinto, M.; Gottlieb, O. Hepatoprotective activity of xanthones and xanthonolignoids against tert-butylhydroperoxide-induced toxicity in isolated rat hepatocytes—comparation with silybin. Pharm. Res. 12 (11): 1756–1760; 1995.
Gorbunov, N. V.; Tyurina, Y. Y.; Salama, G.; Day, B. W.; Claycamp, H. G.; Argyros, G.; Elsayed, N. M.; Kagan, V. E. Nitric oxide protects cardiomyocytes against tert-butyl hydroperoxide-induced formation of alkoxyl and peroxyl radicals and peroxidation of phosphatidylserine. Biochem. Biophys. Res. Commun. 244 (3): 647–651; 1998.
Guennec, J.-Y. L.; Peinau, N.; Esnard, F.; Lacampagne, A.; Gannier, F.; Argibay, J.; Gauthier, F.; Garnier, D. A simple method for calibrating collagenase/pronase E ratio to optimize heart cell isolation. Biol. Cell 79: 161–165; 1993.
Haworth, R.; Goknur, A.; Cook, M.; Decker, R. Use of isolated adult, myocytes to evaluate cardiotoxicity. I. Sugar uptake and protein synthesis. Toxicol. Pathol. 18 (4) (Part 1): 511–520; 1990.
Haworth, R.; Hunter, D.; Berkoff, H. The isolation of Ca2+-resistent myocytes from the adult rat. J. Mol. Cell. Cardiol. 12: 715–723; 1980.
Jewell, S. A.; Monte, D. D.; Richelmi, P.; Bellomo, G.; Orrenius, S. tert-Butylhydroperoxide-induced toxicity in isolated hepatocytes: contribution of thiol oxidation and lipid peroxidation. J. Biochem. Toxicol. 1 (3): 13–22; 1986.
Joyeux, M.; Rolland, A.; Fleurentin, J.; Moriter, F.; Dorfman, P. tert-butyl hydroperoxide-induced injury in isolated rat hepatocytes: a model for studying anti-hepatoxic crude drugs. Planta Med. 56: 171–174; 1990.
Kramer, J. H.; Chovan, J. P.; Schaffer, S. W. Effect of taurine on calcium paradox and ischemic heart failure. Am. J. Physiol. 240: H238-H246; 1981.
Modéus, P.; Hogberg, J.; Orrenius, S. Isolation and use of liver cells. Methods Enzymol. 52: 60–72; 1978.
Pascarel, C.; Brette, F.; Cazorla, O.; Guennec, J. Y. L. Effects on L-type calcium current of agents interfering with the cytoskeleton of isolated guinea-pig ventricular myocytes. Exp. Physiol. 84 (6): 1043–1050; 1999.
Pascarel, C.; Cazorla, O.; Guennec, J. Y. L.; Orchard, C. H.; White, E. Different effects of gadolinium on I(KR), I(KS) and I(K1) in guinea-pig isolated ventricular myocytes. Br. J. Pharmacol. 124 (2): 356–360; 1998.
Ramos, K.; Acosta, D. Application of in vitro model systems to the study of cardiovascular toxicity. In: Gad, S. C., ed. In vitro toxicology. New York: Raven Press; 1994: 221–230.
Remião, F.; Carmo, H.; Carvalho, F.; Bastos, M. L. Inhibition of glutathione reductase by isoproterenol oxidation products. J. Enzyme Inhibition 15: 47–61; 1999.
Remião, F.; Carmo, H.; Carvalho, F.; Bastos, M. L. Simultaneous determination of reduced and oxidized glutathione in freshly isolated rat hepatocytes and cardiomyocytes by HPLC with electrochemical detection. Biomed. Chromatogr. 14: 468–473; 2000.
Shertzer, H. G.; Bannenberg, G. L.; Zhu, H.; Liu, R.-M.; Moldéus, P. The role of thiols in mitochondrial susceptibility to iron and tert-butyl hydroperoxide-mediated toxicity in cultured mouse hepatocytes. Chem. Res. Toxicol. 7: 358–366; 1994.
Suleiman, M.-S. New concepts in the cardioprotective action of magnesium and taurine during the calcium paradox and ischaemia of the heart. Magnesium Res. 7: 295–312; 1994.
Suleiman, M.-S.; Chapman, R. A. Changes in the principal free intracellular amino acids in the langendorf perfused guinea pig heart during arrest with calcium-free or high potassium media. Cardiovasc. Res. 27: 1810–1814; 1993.
Timerman, A. P.; Altschuld, R. A.; Hohl, C. M.; Brierley, G. P.; Merola, A. J. Cellular glutathione and the response of adult rat heart myocytes to oxidant stress. J. Mol. Cell. Cardiol. 22 (5): 565–575; 1990.
Viko, H.; Osnes, J.-B.; Sjetnan, A. E.; Skomedal, T. Improved isolation of cardiomyocytes by trypsination in addition to collagenase treatment. Pharmacol. Toxicol. 76: 68–71; 1995.
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Remião, F., Carmo, H., Carvalho, F. et al. Cardiotoxicity studies using freshly isolated calcium-tolerant cardiomyocytes from adult rat. In Vitro Cell.Dev.Biol.-Animal 37, 1–4 (2001). https://doi.org/10.1290/1071-2690(2001)037<0001:CSUFIC>2.0.CO;2
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DOI: https://doi.org/10.1290/1071-2690(2001)037<0001:CSUFIC>2.0.CO;2